Precision-cut intestinal slices: alternative model for drug transport, metabolism, and toxicology research
نویسندگان
چکیده
منابع مشابه
Cryopreservation of precision-cut tissue slices for application in drug metabolism research.
Cryopreservation of tissue slices greatly facilitates their use in drug metabolism research, leading to efficient use of human organ material and a decrease of laboratory animal use. In the present review, various mechanisms of cryopreservation such as equilibrium slow freezing, rapid freezing and vitrification, and their application to cryopreservation of tissue slices are discussed as well as...
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Predictive in vitro methods to investigate drug metabolism in the human intestine using intact tissue are of high importance. Therefore, we studied the metabolic activity of human small intestinal and colon slices and compared it with the metabolic activity of the same human intestinal segments using the Ussing chamber technique. The metabolic activity was evaluated using substrates to both pha...
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Induction of drug enzyme activity in the intestine can strongly determine plasma levels of drugs. It is therefore important to predict drug-drug interactions in human intestine in vitro. We evaluated the applicability of human intestinal precision-cut slices for induction studies in vitro. Morphological examination and intracellular ATP levels indicated tissue integrity up to 24 h of incubation...
متن کاملCharacterization of rat small intestinal and colon precision-cut slices as an in vitro system for drug metabolism and induction studies.
The aim of this study was to characterize rat small intestinal and colon tissue slices as a tool to study intestinal metabolism and to investigate gradients of drug metabolism along the intestinal tract as well as drug-induced inhibition and induction of biotransformation. Tissue morphology and the intestinal mucus layer remained intact in small intestinal and colon slices during 3 h of incubat...
متن کاملCharacterisation of rat small intestinal and colon precision - cut slices as an in vitro system for drug metabolism and induction studies
This article has not been copyedited and formatted. The final version may differ from this version. Abstract: The aim of this study was to characterise rat small intestinal and colon tissue slices as a tool to study intestinal metabolism and to investigate gradients of drug metabolism along the intestinal tract as well as drug-induced inhibition and induction of biotransformation. Tissue morpho...
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ژورنال
عنوان ژورنال: Expert Opinion on Drug Metabolism & Toxicology
سال: 2016
ISSN: 1742-5255,1744-7607
DOI: 10.1517/17425255.2016.1125882